Warsztaty Single Cell Sequencing

W dniach 7-8.11.2018 w IGC PAN odbędą się warsztaty „Single-Cell Sequencing”Agenda:

Wednesday 7.11.2018

PART I: Lectures (OPEN)

9:00 Welcome

9:05 Introduction to Single Cell Sequencing (dr Agnieszka Ciesielska, BioRad Polska)

9:15 Single cell approach (dr Eddy can Collenburg, Regional Specialist Bio Rad)

9:45 Sequencing Solutions (dr Marta Walczak, OpenExome)

10:15 Break

PART II: Hands-on Workshop (PRE-REGISTRATION REQUIRED)

10:30-12:30 Cells (Cells preparation; Barcode suspension mixes preparation; Single cell isolation)

12:30-15:30 Reverse Transcription (Reverse Transcription Reaction; Emulsion breaking; First Strand Cleaning Up; Second Strand cDNA Synthesis)

Thursday 8.11.2018

PART II: Hands-on Workshop (continued)

9:00-10:30 cDNA cleaning and tagging

10:30-11:00 cDNA amplification

11:00-15:00 Post PCR steps (Library cleaning up; Library checking; Sequencing preparation

 

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