{"id":20588,"date":"2023-03-10T09:08:43","date_gmt":"2023-03-10T09:08:43","guid":{"rendered":"https:\/\/igcz.poznan.pl\/?p=20588"},"modified":"2023-03-10T10:51:50","modified_gmt":"2023-03-10T10:51:50","slug":"8-2023-igc-psd-announcement-concerning-recruitment-to-the-poznan-doctoral-school-of-the-institutes-of-the-polish-academy-of-sciences-pds-ipas-as-part-of-a-research-project","status":"publish","type":"post","link":"https:\/\/igcz.poznan.pl\/en\/open-positions\/8-2023-igc-psd-announcement-concerning-recruitment-to-the-poznan-doctoral-school-of-the-institutes-of-the-polish-academy-of-sciences-pds-ipas-as-part-of-a-research-project\/","title":{"rendered":"[8\/2023\/IGC\/PSD] Announcement concerning recruitment to the Pozna\u0144 Doctoral School of the Institutes of the Polish Academy of Sciences (PDS IPAS) as part of a research project"},"content":{"rendered":"\n

The Director of the Institute of Human Genetics, Polish Academy of Sciences (IHG PAS), and leader of\u00a0 the current research project, Kamila Kusz-Zamelczyk, PhD<\/strong> gives notice of an open competition to be held for the position of PhD student-scholarship holder at the Poznan Doctoral School of Institutes PAS<\/strong>, Independent Research Group of RNA Biology IHG PAS<\/strong><\/p>\n

Number of vacancies: 1<\/strong><\/p>\n

I. General information<\/strong><\/p>\n

    \n
  1. Department in which candidate would work: Independent Research Group of RNA Biology<\/strong><\/li>\n
  2. Discipline: Medical Science<\/strong><\/li>\n
  3. Planned remuneration: scholarship to the value of about 4300 PLN gross (3800 PLN net\/per month) <\/strong><\/li>\n
  4. Period of involvement in research project: 39 months (extension possible)<\/strong><\/li>\n
  5. Deadline for submission of documents: 14.04.2023 r.<\/strong><\/li>\n
  6. Date of announcement: 10.03.2023 r. <\/strong><\/li>\n<\/ol>\n

    The proposed study will be carried out within the OPUS-22 <\/strong>2021\/43\/B\/NZ3\/01635<\/p>\n

    \u00a0<\/strong>Project title: \u201cThe role of NANOS3 in modulating genetic processes of early human germ cell development and implication in the human reproductive health\u201d<\/em><\/strong><\/p>\n

    Research Description:<\/strong><\/p>\n

    Infertility affects approximately 15% of couples worldwide. They are often caused by genetic defects that interfere with the development of germ cells, which in turn is a risk factor for gonadal cancer. NANOS3 is an RNA-binding protein (RBP) that regulates gene expression at the post-transcriptional level and has been identified as a marker of germ cells since their specification during early embryogenesis. However, the precise role of NANOS3 in these early stages is not well understood, and the RNAs it regulates at this stage remain unidentified.<\/p>\n

    \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 The aim of this project is to identify the RNAs and pathways regulated by NANOS3 in the early stages of germ cell development, especially those that are impacted by the infertility-causing NANOS3 mutation p.Glu120Lys (v-NANOS3). Specific objectives are as follows: 1.<\/strong> Creation two types of cell lines, one with degradable NANOS3 protein (degron) using CRISPR-Cas9 and the PiggyBac transposon system, and the other containing the v-NANOS3 mutation using CRISPR-Cas9, and differentiate them into primordial germ cells (PGCs) for further study. 2.<\/strong> Identification of genes which expression is regulated by NANOS3 and v-NANOS3 at early stages of germ cell development using whole transcriptome RNA sequencing (RNA-Seq). 3.<\/strong> Identification of RNAs directly regulated by NANOS3 compared to v-NANOS3 using eCLIP and Nanopore sequencing. 4.<\/strong> Confirmation of the regulation and interaction of NANOS3 with selected most interesting RNAs by RT-qPCR, western blot, immunofluorescence, EMSA.<\/p>\n

    Keywords: <\/strong><\/p>\n

    infertility, germ cell tumor, human germ cell development, RNA-binding proteins, post-transcriptional gene expression regulation, ribonucleoprotein complexes<\/p>\n

    Predicted tasks in the project:<\/strong><\/p>\n