{"id":21622,"date":"2023-07-21T08:09:11","date_gmt":"2023-07-21T08:09:11","guid":{"rendered":"https:\/\/igcz.poznan.pl\/?p=21622"},"modified":"2023-07-21T08:16:08","modified_gmt":"2023-07-21T08:16:08","slug":"23-2023-igc-psd-announcement-concerning-recruitment-to-the-poznan-doctoral-school-of-the-institutes-of-the-polish-academy-of-sciences-pds-ipas-as-part-of-a-research-project","status":"publish","type":"post","link":"https:\/\/igcz.poznan.pl\/en\/open-positions\/23-2023-igc-psd-announcement-concerning-recruitment-to-the-poznan-doctoral-school-of-the-institutes-of-the-polish-academy-of-sciences-pds-ipas-as-part-of-a-research-project\/","title":{"rendered":"[23\/2023\/IGC\/PSD] Announcement concerning recruitment to the Pozna\u0144 Doctoral School of the Institutes of the Polish Academy of Sciences (PDS IPAS) as part of a research project"},"content":{"rendered":"\n

The Director of the Institute of Human Genetics, Polish Academy of Sciences (IHG PAS), and leader of\u00a0 the current research project, Maciej Giefing, professor PhD<\/strong>\u00a0 gives notice of an open competition to be held for the position of PhD student-scholarship holder at the Poznan Doctoral School of Institutes PAS<\/strong>, Department of Cancer Genetics IHG PAS<\/strong><\/p>\n

<\/p>\n

Number of vacancies: 1<\/strong><\/p>\n\n\n\n

I. General information<\/strong><\/p>\n

    \n
  1. Department in which candidate will work: Department of Cancer Genetics<\/strong><\/li>\n
  2. Discipline: Medical Science<\/strong><\/li>\n
  3. Planned remuneration: scholarship to the value of 4300 PLN gross\/per month (3800 PLN net \/per month) <\/strong><\/li>\n
  4. Period of involvement in research project: 15 months (extension possible)<\/strong><\/li>\n
  5. Deadline for submission of documents: 03.09.2023 r.<\/strong><\/li>\n
  6. Date of announcement: 21.07.2023 r.\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/strong><\/li>\n<\/ol>\n

    The proposed study will be carried out within the OPUS-21 <\/strong>2021\/41\/B\/NZ2\/01235
    PI \u2013 Maciej Giefing, professor PhD <\/strong>Project title: \u201cBCOR alterations and the activation of CXCL12 oncogenic pathway <\/em><\/strong>in classical Hodgkin lymphoma\u201d<\/em><\/strong><\/p>\n

      \n
    1. Description of research:<\/strong><\/li>\n<\/ol>\n

      \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 In our previous study we reported recurrent alterations of BCOR, a transcriptional repressor and component of the Polycomb repressive complex PRC1.1, in classical Hodgkin lymphoma (cHL). Further analyzes performed prior to this application triggered the hypothesis that there is a mechanistic link between BCOR loss and the activation of the oncogenic CXCL12 chemokine in cHL.\u00a0 We think that loss of BCOR triggers an important CXCL12-based oncogenic and microenvironment modulating pathway in the pathogenesis of cHL.<\/p>\n

      \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Therefore the aim of this project is the functional validation whether BCOR loss-of-function alterations result in changes in chromatin accessibility and subsequent transcriptional activation of CXCL12. Moreover, we will focus on its effect on cell viability and the tumor microenvironment. We will use advanced molecular tools including CRISPR\/Cas9 genome editing to establish BCOR knock-out cell lines and ATAC-seq to analyze if BCOR loss in the edited cell lines resulted in open chromatin of the CXCL12 promoter region. Moreover, we will use several functional assays like cell viability or chemotaxis assay to analyze for changed chemoattractive properties of the edited CXCL12 expressing cell lines versus non-edited control cell lines. Last we will perform sequencing of microdissected Hodgkin and Reed-Sternberg cells to screen for potential BCOR inactivating mutations in primary cHL cases.<\/p>\n

      \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 In conclusion, in this project we will decipher the mechanism of CXCL12 activation in cHL and show the contribution of this chemokine in the formation of the unique microenvironment in cHL, crucial element of HRS cell survival. The project will be realized in collaboration with German and US research groups.<\/p>\n

      Keywords:<\/strong>\u00a0<\/p>\n

      classical Hodgkin lymphoma, Polycomb proteins, PRC1.1, BCOR<\/em>, activation of the CXCL12 oncogenic<\/p>\n

      pathway, tumor microenvironment<\/p>\n

      Predicted tasks in the project:<\/strong><\/p>\n