Recruitment results:

Recruitment result in competition No. 06/2020/IGC/PSD to Poznań Doctoral School of the Institutes of the Polish Academy of Sciences
Ranking list of the competition No. 06/2020/IGC/PSD

Recruitment result in competition No. 11/2020/IGC/PSD to Poznań Doctoral School of the Institutes of the Polish Academy of Sciences
Ranking list of the competition No. 11/2020/IGC/PSD

Recruitment result in competition No. 16/2020/IGC/PSD to Poznań Doctoral School of the Institutes of the Polish Academy of Sciences
Ranking list of the competition No. 16/2020/IGC/PSD

Recruitment announcements:

  1. [6/2020/IGC/PSD] Announcement about the recruitment to Poznań Doctoral School of the Institutes of the Polish Academy of Sciences (PDS IPAS) at the IHG PAS in Poznań
  2. [11/2020/IGC/PSD] Announcement about the recruitment to Poznań Doctoral School of the Institutes of the Polish Academy of Sciences (PDS IPAS) at the IHG PAS in Poznań
  3. [16/2020/IGC/PSD] Announcement about the recruitment to Poznań Doctoral School of the Institutes of the Polish Academy of Sciences (PDS IPAS) at the IHG PAS in Poznań

[6/2020/IGC/PSD] Announcement concerning  recruitment to the Poznań Doctoral School of the Institutes of the Polish Academy of Sciences (PDS IPAS) as part of a research project

 

The Director of the Institute of Human Genetics, Polish Academy of Sciences (IHG PAS),
and leader of  the current research project, Prof. Jadwiga Jaruzelska
gives notice of an open competition to be held for the position of
PhD student-scholarship holder at the Poznan Doctoral School of Institutes PAS,
Department of Nucleic Acids Genetics IHG PAS

Number of vacancies: 1

I. General information

  1. Department in which candidate would work: Department of Nucleic Acids
  2. Discipline: Medical Science
  3. Planned remuneration: scholarship to the value of 3700 PLN net/ per month extending over 4 years
  4. Deadline for submission of documents: 31.08.2020;
  5. The proposed study will be carried out within the OPUS18 (2019/35/B/NZ1/01665); PI – Prof. Jadwiga Jaruzelska. Project title: NANOS1 RNP-interactome: structure and dynamics during specification/early stages of human germ cell development – significance for human reproduction”.

Concise description of research: Infertility affects 15% of couples world-wide. Genetic defects account for 15-30% of male infertility and are risk factor for the testis germ cell tumour (TGCT). NANOS is post-transcriptional gene expression regulator containing a highly conserved RNA-binding domain composed of a (CCHC)2 zinc-finger, which interacts with a few proteins. We have also identified a v-NANOS1 protein variant associated with infertility in patients.  The general objective now is to obtain a comprehensive insight into the structure and dynamics of NANOS1 ribonucleoprotein interactome during the specification/early stages of human male germ cell development. We will make use of human models with a stable wt- and v-NANOS1 overexpression: 1/ hESCs in vitro differentiation towards specification and early development of primordial germ cells (hPGCLCs) will be performed and 2/ a TCam-2 cell line originating from seminoma and representing a slightly later PGC developmental stage will be used. Our specific objectives are: 1. Identification of target RNAs bound by wt- and v-NANOS1 at different developmental stages by applying protein-crosslinked RNA extraction (XRNAX) and eCLIP. The anti-FLAG antibody will serve to co-IP NANOS1-bound RNAs. Following this, the/a total eCLIP isolated RNA using RiboZero will be analyzed by applying RNA-Seq. Databases, and bioinformatic tools will be used for gene identification. 2. Screening for wt and v-NANOS1 protein binding partners by using Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC) followed by LC-MS/MS mass spectrometry and validation by co-IP followed by western. 3. Identification of specific motifs in target RNAs recognized by wt- and v-NANOS1 protein partners containing RNA-binding domains employing bioinformatic tools and validating RNA-protein interactions by applying luciferase reporter assays. Ribonucleoprotein networks will be assembled by implementing pertinent bioinformatic tools/platforms such as CytoScape. We expect these networks to be at least partially different for wt- and v-NANOS1 in subsequent specific developmental stages. 4. The most interesting interactions (RNA/protein and protein/protein) in the wt-NANOS1 associated network, missing from the v-NANOS1 ones, will be validated by applying phenotypic assays for functional dependence on NANOS1. For this purpose, endogenous NANOS1 silencing will be followed by measurement of the level of NANOS1 targets by real time qPCR/western. We anticipate that the level of target RNAs/protein will change on NANOS1 silencing. If this is so, we will then test whether it influences apoptosis, cell cycle, proliferation or other parameters which could explain the phenotype of the patients and the TCam-2 cells.

        Keywords: infertility, germ cell tumor, human germ cell development, RNA-binding proteins, post-transcriptional gene expression regulation, ribonucleoprotein complexes

Predicted tasks in the project:

– active participation in the realization of project goals.

– supervision of Master’s students.

– participation in writing scientific papers; presenting results at seminars and conferences.

Opportunities

–      work in an international research team, highly experienced in many cutting edge molecular methodologies, and enthusiastic about conducting scientific research

–      research training (courses);

–      participation in international conferences and workshops

II. Requirements for candidates

  1. Master’s degree in molecular biology, biotechnology, genetics, medicine or related field.
  2. Background in molecular biology.
  3. Experience in RNA, DNA, cell culture and molecular biology techniques.
  4. Very good written and oral communication skills in English.
  5. Motivation and enthusiasm about working in the field of science
  6. Good collaborative and team work skills.

III. Required documents

  1. CV, including research achievements.
  2. Cover letter.
  3. A copy of the diploma confirming completion of a Master’s Studies Programme, or a certificate of their completion (in the case of diplomas issued by foreign institutions , the diploma referred to in article 326 para.2 point 2 or article 327 para. 2 of the Act of 20 July 2018 – Law on Higher Education and Science (Journal of Laws of 2018,  item 1668 as amended), giving the right to apply for a doctoral degree in the country in which the University of Higher Education issuing the diploma operates. If the candidate does not have the above-mentioned documents, s/he is obliged to provide them before being admitted to Poznań Doctoral School IPAS. More information about foreign diplomas is available at: https://nawa.gov.pl/uznawalnosc/kontynuacja-nauki-w-polsce/studia-doktoranckie-i-otwieranie-przewodow-doktorskich.
  4. Contact details of at least one current supervisor or other researcher who has previously agreed to issue an opinion about the candidate. The opinion should not be included in the application.
  5. Consent for the processing of candidate’s personal data for the purposes of the recruitment process: http://bip.igcz.poznan.pl/wp-content/uploads/2018/10/Zgoda-rekrutacja-Consent_for_the_processing.pdf
  6. Application for admission to the Poznań Doctoral School IPAS, together with a consent to the processing of personal data for the purposes of the recruitment procedure plus a statement on his/her familiarity with recruitment regulations for the Poznań Doctoral School (PL, EN).
  7. Certificates or other documents indicating level of English language proficiency, if the candidate possesses any.

IV. Criteria for the evaluation of candidates

  1. Candidate’s scientific and professional experience based on his/her participation in conferences, workshops, training courses and internships; participation in research and commercial projects; involvement in scientific societies and associations; international and professional mobility; experience in other sectors, including industry
  2. Background in molecular biology
  3. Candidate’s scientific achievements, based on study grades, scientific and popular science publications, scholarships; prizes and awards resulting from research carried out; student activity or other achievements
  4. Communication skills in English.

V. Announcement of results

Up to 30 days after the deadline of documents submission. Selected candidates will be invited for interview.

VI. Additional conditions

  1. Period of involvement in research project: 10.2020.-31.09.2024
  2. A condition of involvement in the project is participation in the International Doctoral School at IGC PAN (after passing the recruitment procedure). Details of the studies are available on http://igcz.poznan.pl/en/scientific-activity/phd-studies). Fulfillment of  requirements as  set out in the Regulations for Granting Scholarships in Research Grants Financed by the National Research Center are available on (https://www.ncn.gov.pl/sites/default/files/pliki/uchwaly-rady/2019/uchwala25_2019-zal1_ang.pdf).

VII. Additional information

Address to which documents should be submitted, in person or by registered mail: Institute of Human Gentics PAS, ul. Strzeszyńska 32, 60-479 Poznań with postscript [6/2020/IGC/PSD] or by e-mail to the Secretary for Scientific Purposes, phdstudies@igcz.poznan.pl. In the title please include the number of the announcement:  [6/2020/IGC/PSD]

Additional information is available from:jadwiga.jaruzelska@igcz.poznan.pl. The Secretary for Scientific purposes:  phdstudies@igcz.poznan.pl, tel. 61 657 9142

Incomplete applications will not be considered.

Once the recruitment process is finished, unsuccessful candidates will be informed about the scores they have obtained at each step of evaluation.

Refusal of admission to PDS IPAS takes place by way of an administrative decision. The candidate is entitled to submit a request for reconsideration of the decision to the director of the institute concerned


[11/2020/IGC/PSD] Announcement about the recruitment to Poznań Doctoral School of the Institutes of the Polish Academy of Sciences (PDS IPAS) at the IHG PAS in Poznań

The Director of the Institute of Human Genetics, Polish Academy of Sciences (IHG PAS),
and leader of  the current research project, dr Katarzyna Iżykowska
gives notice of an open competition to be held for the position of
PhD student-scholarship holder at the Poznan Doctoral School of Institutes PAS,
Department of Molecular Pathology IHG PAS

Number of vacancies: 1

I. General information

  1. Department in which candidate would work: Department of Molecular Pathology IHG PAS
  2. Discipline: Medical Science
  3. Planned remuneration: scholarship to the value of 3 700 PLN net/ per month
  4. Deadline for submission of documents: 31.08.2020;
  5. 5. Date of announcement: 29.06.2020;
  6. The proposed study will be carried out within the SONATA 15 project (2019/35/D/NZ5/00407); PI – dr Katarzyna Iżykowska; project title: “The role of class II histone deacetylases: HDAC9 and HDAC10 in Sézary syndrome”.

Concise description of research: The aim of this project is to investigate the significance of  HDAC9 and HDAC10 in Sézary syndrome. Sézary syndrome belongs to T-cell cutaneous lymphomas, with still little known etiology, difficult diagnostics and lack of effective treatment. Histone deacetylases are encoded by a group of genes called HDACs, and are involved in epigenetic regulation of gene expression. In the project, effects of chosen HDACs on crucial biological processes like apoptosis, cell cycle, cell proliferation and autophagy will be studied. Moreover, with RNAseq technology global gene expression and cellular pathways affected by those HDACs will be analyzed. Using ChiP-Seq technology binding sites for HDAC9 and HDAC10 in the genome will be mapped and, with co-immunoprecipitation (Co-IP) and mass spectrometry, proteins that are bound to HDACs will be identified. Results will contribute not only to a better understanding of the pathogenesis of Sézary syndrome and perhaps to the improvement of therapy, but also will increase the general knowledge of these histone deacetylase and their mode of action.

Methodology: cell culture, lentiviral transduction, CRISPR-Cas9, immunofluorescence, flow cytometry, RNAseq, Chip-Seq, Co-IP, mass spectrometry

Keywords: Sézary syndrome, histone deacethylases, HDAC9, HDAC10, epigenetic regulation of gene expression, functional studies, next generation sequencing, proteomics.

Predicted tasks in the project:
–        active participation in the realization of project goals.
–        supervision of Master’s students.
–        participation in writing scientific papers; presenting results at seminars and conferences.

Opportunities
–        work in the multidisciplinary research team;
–        research training (courses);
–        participation in conferences

II. Requirements for candidates

  1. Master’s degree in molecular biology, biotechnology, genetics, medicine or related field.
  2. Background in molecular biology.
  3. Experience in RNA, DNA, cell culture and molecular biology techniques.
  4. Very good written and oral communication skills in English.
  5. Motivation and enthusiasm about working in the field of science
  6. Good collaborative and team work skills.

III. Required documents

  1. CV, including research achievements.
  2. Cover letter.
  3. A copy of the diploma confirming completion of a Master’s Studies Programme, or a certificate of their completion (in the case of diplomas issued by foreign institutions , the diploma referred to in article 326 para.2 point 2 or article 327 para. 2 of the Act of 20 July 2018 – Law on Higher Education and Science (Journal of Laws of 2018,  item 1668 as amended), giving the right to apply for a doctoral degree in the country in which the University of Higher Education issuing the diploma operates. If the candidate does not have the above-mentioned documents, s/he is obliged to provide them before being admitted to Poznań Doctoral School IPAS. More information about foreign diplomas is available at: https://nawa.gov.pl/uznawalnosc/kontynuacja-nauki-w-polsce/studia-doktoranckie-i-otwieranie-przewodow-doktorskich.
  4. Contact details of at least one current supervisor or other researcher who has previously agreed to issue an opinion about the candidate. The opinion should not be included in the application.
  5. Consent for the processing of candidate’s personal data for the purposes of the recruitment process: download
  6. Application for admission to the Poznań Doctoral School IPAS, together with a consent to the processing of personal data for the purposes of the recruitment procedure plus a statement on his/her familiarity with recruitment regulations for the Poznań Doctoral School. (download).
  7. Certificates or other documents indicating level of English language proficiency, if the candidate possesses any.

IV. Criteria for the evaluation of candidates

  1. Candidate’s scientific and professional experience based on his/her participation in conferences, workshops, training courses and internships; participation in research and commercial projects; involvement in scientific societies and associations; international and professional mobility; experience in other sectors, including industry
  2. Background in molecular biology
  3. Candidate’s scientific achievements, based on study grades, scientific and popular science publications, scholarships; prizes and awards resulting from research carried out; student activity or other achievements
  4. Communication skills in English.

V. Announcement of results

Up to 30 days after the deadline of documents submission. Selected candidates will be invited for interview.

VI. Additional conditions

  1. Period of involvement in research project: 10.2020 – 31.09.2023
  2. A condition of involvement in the project is participation in the International Doctoral School at IGC PAN (after passing the recruitment procedure). Details of the studies are available on http://igcz.poznan.pl/en/scientific-activity/phd-studies). Fulfillment of requirements as  set out in the Regulations for Granting Scholarships in Research Grants Financed by the National Research Center are available on (https://www.ncn.gov.pl/sites/default/files/pliki/uchwaly-rady/2019/uchwala25_2019-zal1_ang.pdf).

VII. Additional information

Address to which documents should be submitted, in person or by registered mail: Institute of Human Gentics PAS, ul. Strzeszyńska 32, 60-479 Poznań with postscript [11/2020/IGC/PSD] or by e-mail to the Secretary for Scientific Purposes, phdstudies@igcz.poznan.pl. In the title please include the number of the announcement:  [11/2020/IGC/PSD]

Additional information is available from: (katarzyna.izykowska@igcz.poznan.pl). The Secretary for Scientific purposes:  phdstudies@igcz.poznan.pl, tel. 61 657 91 42

 Incomplete applications will not be considered.

Once the recruitment process is finished, unsuccessful candidates will be informed about the scores they have obtained at each step of evaluation.

Refusal of admission to PDS IPAS takes place by way of an administrative decision. The candidate is entitled to submit a request for reconsideration of the decision to the director of the institute concerned.


[16/2020/IGC/PSD] Announcement concerning  recruitment to the Poznań Doctoral School of the Institutes of the Polish Academy of Sciences (PDS IPAS) as part of a research project

The Director of the Institute of Human Genetics, Polish Academy of Sciences (IHG PAS),
and leader of  the current research project, Natalia Rozwadowska PhD
gives notice of an open competition to be held for the position of
PhD student-scholarship holder at the Poznan Doctoral School of Institutes PAS,
Department of Molecular Pathology IHG PAS

Number of vacancies: 1

I. General information

  1. Department in which candidate would work: Department of Molecular Pathology
  2. Discipline: Medical Science
  3. Planned remuneration: scholarship to the value of about 3 800 PLN net/ per month (1-24 months); 4 500 PLN net (25-48 month)
  4. Deadline for submission of documents: 29.08.2020;
  5. 5. Date of announcement: 29.07.2020;
  6. The proposed study will be carried out within the PRELUDIUM BIS 2019/35/O/NZ5/01900; PI – Natalia Rozwadowska, PhD

Project title: The survey of genes regulated by RBM protein family member – a component of splicing machinery in human spermatogenesis

        Concise description of research:

Human spermatogenesis is a multistep, complex process and requires plenty of factors cooperating together. In modern world, male infertility is an increasing worldwide problem, which encourages researchers to study molecular background of spermatozoa formation.
One of the major obstacles to investigate spermatogenesis is a lack of well-established, easily accessible model. Nowadays, studies concerning gametogenesis are conducted based on stem cells models in specialized scientific institutions.
In proposed project we aim to perform enhanced survey of genes regulated by new, possibly related to spermatogenesis failure RBMXL proteins using in vitro seminoma model: Tcam-2 cell line as well as primordial germ cell-like cells (PGC-LCs) derived from human embryonic stem cells (hESC).
Our and other group results indicate the RBMXL proteins plays role in RNA metabolism, in particular, in splicing related processes such as Exon Junction Complex assembly on pre-mRNA molecule and Nonsense mediated decay. Hence, we would like to enhance the survey of RBMXL proteins using cutting edge RIPseq, eCLIP-seq (enhanced crosslinked and immunoprecipitation followed by sequencing; NGS ), RNAseq technology and uncover genes regulated by this protein with emphasis on their differentially expressed splicing forms. Moreover, we have a unique opportunity to validate our results using stem cell-based model to study human germ cell development

        Keywords: spermatogenesis, stem cell, RBM protein family, RNA metabolism

Predicted tasks in the project:

– active participation in the realization of project goals.
– supervision of Master’s students.
– participation in writing scientific papers; presenting results at seminars and conferences.

Opportunities

– work in an international research team, highly experienced in many cutting edge molecular and cellular methodologies, and enthusiastic about conducting scientific research
– research training (courses);
– participation in international conferences and workshops
– NAWA scholarship for  6-month long scientific stay (additional 9000 PLN/month stipend)

II. Requirements for candidates

  1. Master’s degree in molecular biology, biotechnology, genetics, medicine or related field.
  2. Background in molecular biology.
  3. Experience in RNA, DNA, cell culture and molecular biology techniques.
  4. The bioinformatic skills will be an advantage
  5. Very good written and oral communication skills in English.
  6. Motivation and enthusiasm about working in the field of science
  7. Good collaborative and team work skills.

III. Required documents

  1. CV, including research achievements.
  2. Cover letter.
  3. A copy of the diploma confirming completion of a Master’s Studies Programme, or a certificate of their completion (in the case of diplomas issued by foreign institutions , the diploma referred to in article 326 para.2 point 2 or article 327 para. 2 of the Act of 20 July 2018 – Law on Higher Education and Science (Journal of Laws of 2018,  item 1668 as amended), giving the right to apply for a doctoral degree in the country in which the University of Higher Education issuing the diploma operates. If the candidate does not have the above-mentioned documents, s/he is obliged to provide them before being admitted to Poznań Doctoral School IPAS. More information about foreign diplomas is available at: https://nawa.gov.pl/uznawalnosc/kontynuacja-nauki-w-polsce/studia-doktoranckie-i-otwieranie-przewodow-doktorskich.
  4. Contact details of at least one current supervisor or other researcher who has previously agreed to issue an opinion about the candidate. The opinion should not be included in the application.
  5. Consent for the processing of candidate’s personal data for the purposes of the recruitment process: http://bip.igcz.poznan.pl/wp-content/uploads/2018/10/Zgoda-rekrutacja-Consent_for_the_processing.pdf
  6. Application for admission to the Poznań Doctoral School IPAS, together with a consent to the processing of personal data for the purposes of the recruitment procedure plus a statement on his/her familiarity with recruitment regulations for the Poznań Doctoral School (download).
  7. Certificates or other documents indicating level of English language proficiency, if the candidate possesses any.

IV. Criteria for the evaluation of candidates

  1. Candidate’s scientific and professional experience based on his/her participation in conferences, workshops, training courses and internships; participation in research and commercial projects; involvement in scientific societies and associations; international and professional mobility; experience in other sectors, including industry
  2. Background in molecular biology
  3. Candidate’s scientific achievements, based on study grades, scientific and popular science publications, scholarships; prizes and awards resulting from research carried out; student activity or other achievements
  4. Communication skills in English.

V. Announcement of results

Up to 30 days after the deadline of documents submission. Selected candidates will be invited for interview.

VI. Additional conditions

  1. Period of involvement in research project: 10.2020-31.09.2023 (36 months)
  2. A condition of involvement in the project is participation in the International Doctoral School at IGC PAN (after passing the recruitment procedure). Details of the studies are available on http://igcz.poznan.pl/en/scientific-activity/phd-studies). Fulfillment of requirements as  set out in the Regulations for Granting Scholarships in Research Grants Financed by the National Research Center are available on (https://www.ncn.gov.pl/sites/default/files/pliki/uchwaly-rady/2019/uchwala25_2019-zal1_ang.pdf).

VII. Additional information

Address to which documents should be submitted, in person or by registered mail: Institute of Human Gentics PAS, ul. Strzeszyńska 32, 60-479 Poznań with postscript [16/2020/IGC/PSD] or by e-mail to the Secretary for Scientific Purposes, phdstudies@igcz.poznan.pl. In the title please include the number of the announcement:  [16/2020/IGC/PSD]

Additional information is available from: Natalia Rozwadowska: natalia.rozwadowska@igcz.poznan.pl The Secretary for Scientific purposes:  phdstudies@igcz.poznan.pl, tel. 61 657 91 42

Incomplete applications will not be considered.

Once the recruitment process is finished, unsuccessful candidates will be informed about the scores they have obtained at each step of evaluation.

Refusal of admission to PDS IPAS takes place by way of an administrative decision. The candidate is entitled to submit a request for reconsideration of the decision to the director of the institute concerned.