The department carries out studies into the molecular background of male infertility and the biological properties of stem cells (of myogenic origin) from the perspective of their application in organ regeneration. We apply the tools of systemic biology to the transcriptomic identification of gene expression in dysfunctional male gonads and DNA arrays of infertile individuals to identify novel genes. In the second module, studies of pro-generative properties of stem cells are investigated in clinical trials for regeneration of a) post-infarction heart, b) anal sphincters and c) muscular dystrophies.
Department of Reproductive Biology and Stem Cells
Featured publications
Variants in GCNA, X-linked germ-cell genome integrity gene, identified in men with primary spermatogenic failure.
Hardy JJ, Wyrwoll MJ, Mcfadden W, Malcher A, Rotte N, Pollock NC, Munyoki S, Veroli MV, Houston BJ, Xavier MJ, Kasak L, Punab M, Laan M, Kliesch S, Schlegel P, Jaffe T, Hwang K, Vukina J, Brieño-Enríquez MA, Orwig K, Yanowitz J, Buszczak M, Veltman JA, Oud M, Nagirnaja L, Olszewska M, O’Bryan MK, Conrad DF, Kurpisz M, Tüttelmann F, Yatsenko AN; GEMINI Consortium
Hum Genet, 2021 Aug;140 (8):1169-1182. doi: 10.1007/s00439-021-02287-y. Epub 2021 May 7.IF-4,132 MNiSW-100
Addition of Popular Exogenous Antioxidant Agent, PBN, to Culture Media May Be an Important Step to Optimization of Myogenic Stem/Progenitor Cell Preparation Protocol.
Nowaczyk M, Malcher A, Zimna A, Łabędź W˛ Kubaszewski Ł, Barczak W , Rubiś B, Rozwadowska N and Kurpisz M
Antioxidants (MDPI), 2021, 1 (6), e959, 17 stron, https://doi.org/10.3390/antiox10060959IF-100 MNiSW-6,312
The Negative Impact of Varicocele on Basic Semen Parameters, Sperm Nuclear DNA Dispersion and Oxidation-Reduction Potential in Semen.
Gill K, Kups M, Harasny P, Machalowski T, Grabowska M, Lukaszuk M, Matuszewski M, Duchnik E, Frączek M, Kurpisz M and Piasecka M
Int J Environ Res Public Health (MDPI), 2021, 18 (11): e5957, 16 str, https: / doi.org. 103390.ijerph18115977IF-2,849 MNiSW-70
Effect of acute isooxic hypercapnia on oxidative activity of systemic neutrophils in endotoxemic rabbits.
Billert H, Bednarek E, Kusz K, Ponichter M, Kurpisz M
Cent Eur J Immunol 2021; 46 (1): 47-53 https://doi.org/10.5114/ceji.2021.105245IF-1,415 MNiSW-40
Badanie chromosomów plemnikowych u sześciu nosicieli rzadkich i częstych translokacji robertsonowskich. Publikacja dwujęzyczna (ang. i pol.).
Olszewska M, Wiland E, Wanowska E, Hueluk N, Chernykh VB, Zastavna D, Kurpisz M
Postepy Hig Med Dosw, 2021; 75: 199-210; DOI: 10.5604/01.3001.0014.8122IF-0,879 MNiSW-40
Viability and intracellular nitric oxide generation in the umbilical cord blood CD34+CD133– and CD34+CD133+ cell populations exposed to local anaesthetics.
Kulińska K, Billert H, Sawiński K, Czerniak K, Kurpisz M
Central Eur J Immunol, 2020, 45 (4): 369-376, DOI: https://doi.org/10.5114/ceji.2020.103410IF-1,415 MNiSW-40
Autoimmunologiczne przyczyny niepłodności mężczyzn. Rozdz. 36.
Kamieniczna M, Kurpisz M
W: Andrologia. Zdrowie mężczyzna od fizjologii do patologii pod red. Jolanty Słowikowskiej-Hilczer, PZWL, Warszawa, 2021, str: 639-656, ISBN 978-83-2006-066-9MNiSW-20
Wpływ infekcji na czynność plemników. Rozdz.37.
Fraczek M, Kurpisz M
W: Andrologia. Zdrowie mężczyzny od fizjologii do patologii pod red. Jolanty Słowikowskiej-Hilczer, PZWL, Warszawa, 2021, str. 657-678, ISBN 978-83-2006-066-9MNiSW-20
How much, if anything, do we know about sperm chromosomes of Robertsonian translocation carriers?
Wiland E, Olszewska M, Woźniak T, Kurpisz M
Cell Mol Life Sci, 2020, 77(23): 47-65-4785, Epub 2020 Jun 8, doi: 10.1007/s00018-020-03560-5IF-6,496 MNiSW-140
Transient and Stable Overexpression of Extracellular Superoxide Dismutase Is Positively Associated with the Myogenic Function of Human Skeletal Muscle-Derived Stem/Progenitor Cells.
Nowaczyk M, Malcher A, Zimna A, Łabędź W, Kubaszewski Ł, Fiedorowicz K, Wierzbiński K, Rozwadowska N and Kurpisz M
IF-5,014 MNiSW-100
All publications
Featured research projects
1. National Science Centre research project 2017/26/D/NZ5/00789
“Novel causative genetic variants in azoospermia: whole genome analysis and functional in vitro studies”
Duration: 2018-2021
2. National Science Centre research project 2017/25/B/NZ5/01131
“Genetically corrected autologous myogented with nanocapsules as the next generation formula for treatment of Duchenne disease.”
Duration: 2018-2021
3. National Science Centre research project 2015/17/B/NZ2/01157
“Schearching for novel genes essential for human oligo- i azoospermia – mouse „knockout” model”
Duration: 2016-2020
4. National Science Centre research project 2015/17/D/NZ5/03442
“Analysis of DNA methylation pattern in spermatozoa of infertile men with oligozoospermia”
Duration: 2016-2020
5. National Centre for Research and Development (Applied Research Programme) research project PBS3/A7/27/2015
“Molecular imaging (including nanotechnology) for monitoring of implanted stem cells and their pro-regenerative functions – STEMNanoT”
Duration: 2015 – 2020
6. National Centre for Research and Development (STRATEGMED Programme)research project STRATEGMED1/233209/12/NCBR/2015
“Application of glial progenitors for treatment of ALS – GRP&ALS”
Duratiob: 2015 – 2019
7. National Centre for Research and Development (STRATEGMED Programme) research project STRATEGMED1/233624/5/NCBR/2014
“Low molecular weight epigenetic modulators for activation of pluripotency of cells for regenerative medicine purposes – EPI CELL”
Duration: 2015 – 2019
8. National Science Centre research project 2015/19/B/NZ5/02241
“Verification of the hypothesis of oxidative stress, apoptosis and immune factor as the mechanisms responsible for the poor semen quality in men exposed to genital heat stress”
Duration: 2016-2019
9. National Science Centre research project 2014/15/B/ST4/04946
“Quantification of cancer tissue oxygenation measured with BOLD-MRI – calibration with oximetry EPRI”
Duration: 2015 – 2019
10. National Science Centre research project 2014/13/B/NZ3/04646
“Cytoprotective role of antioxidants towards cells of myogenic origin in in vitro and in situ conditions.”
Duration: 2015 – 2018
Collaborations
Poznan University of Medical Sciences
Nanobiomedical Centre Adam Mickiewicz University In Poznań
University of Warmia and Mazury in Olsztyn
University of Warsaw Biological and Chemical Research Centre
Centre for Postgraduate Medical Education, Warsaw
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań
Institute of Gynaecology and Obstetrics in Poznań
MedPolonia Private Specialist Hospitals, Poznań
Institute of Immunology and Experimental Therapy, Polish Academy of Sciences in Wroclaw
Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw
Jagiellonian Centre for Experimental Therapeutics, Kraków
Second Clinic of General Surgery, Jagiellonian University in Kraków
Johns Hopkins University School of Medicine, Institute of Cell Engineering, Baltimore, MD, USA.
University of Vienna, Vienna, Austria, Cardiac Department, Austria
Freie University, Department of Physics ,Germany
Yuriy Fedkovych Chernivtsi National University, Ukraine
Danylo Halytsky Lviv National Medical University, Department of Paediatric Surgery, Ukraine.
Institute of Hereditary Pathology of National Academy of Medical Sciences of Ukraine, Ukraine
Awards
Awarded:
dr n. med. Marta Olszewska
Early Stage Investigator registration scholarship, American Society of Andrology, 46th Annual Conference
Given by: American Society of Andrology
Early Stage Investigator registration scholarship
Awarded:
dr n. med. Marta Olszewska
Polish Intelligent Development Award 2020 in the category: Scientist of the future
Given by: Intelligent Development Center
Analysis of DNA methylation pattern in spermatozoa of infertile men with oligozoospermia, funded by National science Centre, SONATA 2015/17/D/NZ5/03442.
Awarded:
dr n. med. Marta Olszewska
Nomination for the "AMBASSADOR OF INNOVATION"
Given by: International forum Economic, organized by the European Centre of the development of the economy. http://miedzynarodowe-forum.pl
economic award for thinking ahead, pioneering projects and numerous successes
Awarded:
dr n. med. Marta Olszewska
dr hab. n. med. Monika Frączek
prof. dr hab. n. med. Maciej Kurpisz
dr n. med. Marta Olszewska
dr hab. n. med. Monika Frączek
prof. dr hab. n. med. Maciej Kurpisz
Oustanding Paper Award for the best paper published in 2019 in Asian Journal of Andrology
Given by: Editorial Office of Asian Journal of Andrology (Wolters Kluwer)
Oustanding Paper Award for the best paper published in 2019 in Asian Journal of Andrology Olszewska M, Barciszewska MZ, Fraczek M, Huleyuk N, Chernykh VB, Zastavna D, Barciszewski J, Kurpisz M:
Global methylation status of sperm DNA in carriers of chromosome structural aberrations.
Asian J Androl, 2017, 19: 117-124. https://dx.doi.org/10.4103/1008-682X.168684
Awarded:
dr n. med. Marta Olszewska
Nomination for the Polish Intelligent Development Award 2020 in the category: Sciencist of the future.
Given by: Polish Intelligent Development
Nomination for the Polish Intelligent Development Award 2020 in the category: Sciencist of the future for the project: : “Analysis of DNA methylation pattern in spermatozoa of infertile men with oligozoospermia”. The material regarding the nominee is available on website: https://rzeczo.pl/blog/2020/07/08/nieplodnosc-to-tez-epigenetyka/.
Awarded:
dr hab. n. med. Monika Frączek
Nomination for the Polish Inteligent Develpoment Award 2020
Given by: The center of intelligent development
Nomination for the Polish Intelligent Development Award 2020 in the category: Sciencist of the future for the project: ‘Verification of the hypothesis of oxidative stress, apoptosis and immune factor as the mechanisms responsible for the poor semen quality in men exposed to genital heat stress’. The winners of this prestigious award have been for five years organizations and people who in their work show a constant focus on the future, constituting the basis of sustainable economic development of the country.
Awarded:
dr n. med. Agnieszka Malcher (Wacławska)
Nomination for the Polish Inteligent Development Award 2019
Given by: Centrum for Intelligent Development
Dr n. med. Agnieszka Malcher has been nominated for the Polish Intelligent Development Award 2019 in the category: Scientist of the future for the project: "Novel causative genetic variants in azoospermia: whole genome analysis and functional in vitro studies." The winners of this prestigious award have been for four years organizations and people who in their work show a constant focus on the future, constituting the basis of sustainable economic development of the country.
Awarded:
dr n. med. Agnieszka Malcher (Wacławska)
Short-Term Fellowships
Given by: European Molecular Biology Organization (EMBO).
Short-Term Fellowships of European Molecular Biology Organization (EMBO) to implement the project CRISPR/Cas9 system based spermatogenesis impairment in vitro modeling of human male gonad. The research will be conducted in Max Planck Institute for Molecular Genetics in Germany.
Awarded:
dr n. med. Agnieszka Zimna
Scholarship
Given by: the Foundation for Polish Science
For the 27th time, the Foundation for Polish Science awarded START scholarships to the most talented young scientists from all over Poland. Among 100 young researchers was Dr. Agnieszka Zimna from the Department of Reproductive Biology and Stem Cells.
Awarded:
prof. dr hab. n. med. Maciej Kurpisz
The Ludwik Hirszfeld prize
Given by: Chairman and the Board of Polish Society for Experimental and Clinical Immunology
For his outstanding contribution to development of Immunology in Poland (Warsaw, 8th June, 2017)