W dniach 7-8.11.2018 w IGC PAN odbędą się warsztaty „Single-Cell Sequencing”Agenda:
Wednesday 7.11.2018
PART I: Lectures (OPEN)
9:00 Welcome
9:05 Introduction to Single Cell Sequencing (dr Agnieszka Ciesielska, BioRad Polska)
9:15 Single cell approach (dr Eddy can Collenburg, Regional Specialist Bio Rad)
9:45 Sequencing Solutions (dr Marta Walczak, OpenExome)
10:15 Break
PART II: Hands-on Workshop (PRE-REGISTRATION REQUIRED)
10:30-12:30 Cells (Cells preparation; Barcode suspension mixes preparation; Single cell isolation)
12:30-15:30 Reverse Transcription (Reverse Transcription Reaction; Emulsion breaking; First Strand Cleaning Up; Second Strand cDNA Synthesis)
Thursday 8.11.2018
PART II: Hands-on Workshop (continued)
9:00-10:30 cDNA cleaning and tagging
10:30-11:00 cDNA amplification
11:00-15:00 Post PCR steps (Library cleaning up; Library checking; Sequencing preparation
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